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Controlling the differentiation of human pluripotent stem cells is the goal of many laboratories, both to study normal human development and to generate cells for transplantation. One …
WhatsApp: +86 18221755073
Differentiation between wild and cultivated species, and potentially discrimination of species from different geographical origins could also be achieved based on the DI-MS …
WhatsApp: +86 18221755073
Given the role that nicotinamide has been shown to play in the differentiation of RPE from pluripotent stem cells (Idelson et al., 2009; Maruotti et al., 2015; Saini et al., 2017), we …
WhatsApp: +86 18221755073
The differentiation experiment was replicated three times, and for each cell state, spectra were collected from at least 20 cells. 10 SERS spectra were obtained for each cell by selecting …
WhatsApp: +86 18221755073
We describe a robust method to direct the differentiation of pluripotent stem cells into retinal pigment epithelial cells (RPE). The purpose of providing a detailed and thorough …
WhatsApp: +86 18221755073
It was reported that endothelial cells (CD34+CD31+) were present at day 5 of differentiation, and higher purity was achieved by day 7. Here, the protocol employs a similar approach, but with …
WhatsApp: +86 18221755073
Single-strand conformation polymorphism (SSCP) analysis of ribosomal DNA (rDNA) was investigated for rapid differentiation of phenotypically similar yeast species. Sensitive tests …
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The majority of designed methods for the differentiation of these species are time-consuming, some of them are very high-priced procedures, and a few of them cannot be used …
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Here, we describe a simple protocol to rapidly differentiate ARPE-19 cells, producing homogenous cultures that reacquire many of the characteristics of their in vivo counterparts. We propose …
WhatsApp: +86 18221755073
After obtaining the NPCs, astrocytic differentiation starts anytime thereafter. Astrocyte differentiation was done on Materigel™ coated plates, which was different from the …
WhatsApp: +86 18221755073
Conclusions. Among the tested procedures, rep-PCR proved to be the most effective and reliable method allowing rapid differentiation of Bifidobacterium strains. …
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A multiplex quantitative PCR (qPCR) was developed and evaluated for the simultaneous detection of Salmonella spp., S. enterica serovar Typhimurium and S. enterica serovar Enteritidis in various (food) matrices. …
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The development and validation of a one-step, real-time, and quantitative dengue virus serotype-specific reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay …
WhatsApp: +86 18221755073
The period for the differentiation of mature astrocytes took only 4 weeks from NPCs. We confirmed the maturity of the differentiated astrocytes via the expression of …
WhatsApp: +86 18221755073
Controlling the differentiation of human pluripotent stem cells is the goal of many laboratories, both to study normal human development and to generate cells for …
WhatsApp: +86 18221755073
Loss of RPE differentiation has long been known to play a critical role in numerous retinal diseases, including inherited rod-cone degenerations, inherited macular degeneration, …
WhatsApp: +86 18221755073
The induction of pluripotency in adult human cells provides an alternative cell source, both for in vitro studies and for clinical applications (Takahashi et al., 2007), avoiding …
WhatsApp: +86 18221755073
Controlling the differentiation of human pluripotent stem cells is the goal of many laboratories, both to study normal human development and to generate cells for transplantation. One …
WhatsApp: +86 18221755073
The Lactobacillus species are important groups of bacteria to control human intestinal microflora and preserve it in a normal state (Song et al., 2000).During the past …
WhatsApp: +86 18221755073
Therefore, it is of significance to establish an effective differentiation methodology to generate highly pure OPCs and OLs from some easily accessible stem cell sources. To achieve this …
WhatsApp: +86 18221755073
Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in …
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Long-term culture of hESC-derived motor neurons. a Immunocytochemical analysis of HB9, ISL-1, βIII-Tubulin, and ChAT after 1, 2, or 4 weeks of monolayer differentiation. Scale bar, 100 μm, …
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Unfortunately, the differences in community differentiation among different grazing modes were not significant at the initial stage of grazing, so it was not possible to better …
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Obtaining differentiated cells with high physiological functions by an efficient, but simple and rapid differentiation method is crucial for modeling neuronal diseases in vitro using …
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Differentiation 75: 337–349, 2007 [Google Scholar] 11. Usui J, Kobayashi T, Yamaguchi T, Knisely AS, Nishinakamura R, Nakauchi H: Generation of kidney from pluripotent stem cells via …
WhatsApp: +86 18221755073
A rapid and direct method for differentiation of HN-AD strains based on derivative synchronous fluorescence spectra has been developed: Based on the study of fluorescence …
WhatsApp: +86 18221755073
We have found that the addition of defined factors at specific times leads to conversion of approximately 80% of the cells to an RPE phenotype in only 14 days. This …
WhatsApp: +86 18221755073
We describe a simple, rapid method to establish cultures from ARPE-19 cells, with significantly improved epithelial cell morphology and cytoskeletal organization, and RPE-related functions.
WhatsApp: +86 18221755073
Rapid and accurate identification of Staphylococcus aureus (SA) in blood culture specimens is crucial for timely clinical intervention. Traditional Gram staining methods, …
WhatsApp: +86 18221755073
Purpose : ARPE-19 is an immortalized cell line of retinal pigment epithelium (RPE) currently used to investigate RPE cell biology. Continuous passaging over the past two decades has resulted …
WhatsApp: +86 18221755073
The present study describes the development of a multiplex assay (EFMAtyping) for simultaneous identification of pathogens causing typhoid and paratyphoid fever in a single …
WhatsApp: +86 18221755073
Here, we report a highly efficient system for differentiating human embryonic stem cells and induced pluripotent stem cells (referred to collectively as hPSCs) into cells expressing markers …
WhatsApp: +86 18221755073
Dengue virus is a mosquito-borne flavivirus and the most widely prevalent arbovirus in tropical and subtropical regions of Asia, Africa, and Central and South America ().There are four …
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Human pluripotent stem cells (hPSCs) can generate a diversity of cell types, but few methods have been developed to derive cells of the kidney lineage. Here, we report a highly efficient …
WhatsApp: +86 18221755073
In this study, we present a simple, rapid protocol for differentiation of human iPSCs into functional spinal (lower) MNs, involving only adherent culture and use of small molecules for directed …
WhatsApp: +86 18221755073